Studies on the physicochemical properties of the new preparation deltaferon as a component of a molecular construct

Autor:	M. P. Bogryantseva, O. S. Korovina, G. M. Levagina, V. I. Masycheva, Denis V. Korneev
Rok vydání:	2013
Předmět:	Proteases biology RNA Trypsin Applied Microbiology and Biotechnology Biochemistry Molecular biology law.invention RNA silencing Environmental temperature law biology.protein Recombinant DNA medicine Specific activity Ribonuclease medicine.drug
Zdroj:	Applied Biochemistry and Microbiology. 49:660-663
ISSN:	1608-3024 0003-6838
DOI:	10.1134/s0003683813070041
Popis:	A molecular construct of a spherical shape containing double-stranded RNA (dsRNA) in the center, coated by a spermidine-polyglucine coat, and carrying recombinant deltaferon—an analogue of recombinant interferon-gamma—has been designed. It is shown that deltaferon in this construct retains its specific activity at a level of 9 × 104 U/mg. It was found that deltaferon and dsRNA in the construct were characterized by enhanced resistance to proteases (trypsin) and nucleases (E. coli ribonuclease) as compared to free deltaferon and dsRNA. An increased resistance of deltaferon in the construct to environmental temperature during storage was also demonstrated.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::65b7d9b0ac9fd77407551e766aa5ec28 https://doi.org/10.1134/s0003683813070041 Zobrazit plný text záznamu Plný text ve formátu PDF