Altered distribution of interstitial cells in the guinea pig bladder following bladder outlet obstruction
| Autor: | Yasue Kubota, Kenjiro Kohri, Yoshio Mabuchi, Yoshiyuki Kojima, Hikaru Hashitani, Tsuyoshi Soji, Hikaru Suzuki, Shoichi Sasaki, Nobuyuki Shirasawa |
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| Rok vydání: | 2007 |
| Předmět: |
Pathology
medicine.medical_specialty education.field_of_study biology business.industry Urology Population Connective tissue Vimentin urologic and male genital diseases female genital diseases and pregnancy complications Pathophysiology Guinea pig Bladder outlet obstruction medicine.anatomical_structure medicine biology.protein Immunohistochemistry Distribution (pharmacology) Neurology (clinical) education business |
| Zdroj: | Neurourology and Urodynamics. 27:330-340 |
| ISSN: | 0733-2467 |
| DOI: | 10.1002/nau.20502 |
| Popis: | Aims We investigated the effects of bladder outlet obstruction (BOO) on the distribution of interstitial cells (ICs) in the guinea-pig bladder. Methods Bladder overactivity of BOO animals was validated with urodynamic studies. Immunohistochemical analyses for Kit and vimentin as markers for ICs were performed on both BOO and control bladders. Morphological and functional properties of detrusor smooth muscle (DSM) were examined with α-smooth muscle actin staining and intracellular recording, respectively. Electron microscopy was also carried out to characterize ultrastructural morphology of ICs. Results Two weeks after surgery, BOO animals showed an increased voiding frequency and a reduced voiding volume. Filling cystometry demonstrated a frequent incidence of non-voiding contractions, a reduced interval between voiding contractions and an increased voiding pressure in BOO bladders. In BOO bladders, the thickness of suburothelial and subserosal connective tissue layers was increased, whilst that of detrusor smooth muscle (DSM) layer was less affected. Population of Kit or vimentin immunoreactive ICs was increased in subserosal layers, and their distribution was altered in suburotherial layer in BOO bladders. Neither α-actin immunoreactivity nor spontaneous electrical activity of DSM was altered in BOO bladders. ICs were characterized by their numerous mitochondria and caveolae, and had a close contact with each other and with neighboring DSM or nerves. Conclusions These results demonstrated the increased population of ICs in the BOO guinea-pig model for the first time, and suggest that the altered distribution of ICs may contribute to the pathophysiology of bladder overactivity. Neurourol. Urodynam. 27:330–340, 2008. © 2007 Wiley-Liss, Inc. |
| Databáze: | OpenAIRE |
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