Genetic diversity within the 18S rRNA and actin locus of Cryptosporidium scrofarum (Apicomplexa: Cryptosporidiidae) infecting domestic pigs (Sus scrofa domesticus) of India

Autor:	Harkirat Singh, Devina Sharma, Nirbhay Kumar Singh, Damer P. Blake, Shitanshu S. Rath
Rok vydání:	2021
Předmět:	Genetics Apicomplexa Genetic diversity General Veterinary animal diseases Cryptosporidium scrofarum Cryptosporidiidae Locus (genetics) Biology biology.organism_classification Actin 18S ribosomal RNA
Zdroj:	Veterinarski arhiv. 91:269-276
ISSN:	1331-8055 0372-5480
Popis:	The genetic diversity was studied of Cryptosporidium scrofarum (syn Cryptosporidium pig genotype II) of domestic pigs (Sus scrofa domesticus) from Punjab, India. Nested PCR amplification targeting the 18S rRNA and actin gene loci from Cryptosporidium positive samples was carried out, and the amplicons were sequenced. Phylogenetic comparison of a partial 18S rRNA gene revealed that they were genetically most similar to C. scrofarum isolated from other parts of the world. However, comparison of sequences representing a fragment of the genomic actin locus identified a new genotype conserved within the isolates sampled from India but distinct from other published sequences, suggesting the presence of a different Indian genotype.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::65addddfb23ba8834192b872722914b6 https://doi.org/10.24099/vet.arhiv.0674 Zobrazit plný text záznamu