Evaluation of biodegradation and biocompatibility of collagen/chitosan/alkaline phosphatase biopolymeric membranes
| Autor: | Maria Paraschiv, M Simoiu, R Tatia, A Apatean, Daniela Ionita, Elena Berteanu, Luminita Tcacenco, Manuela Sidoroff |
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| Rok vydání: | 2016 |
| Předmět: |
Materials science
Biocompatibility 02 engineering and technology equipment and supplies 010402 general chemistry 021001 nanoscience & nanotechnology 01 natural sciences 0104 chemical sciences Chitosan chemistry.chemical_compound Membrane Biochemistry chemistry Mechanics of Materials Collagenase medicine Alkaline phosphatase General Materials Science MTT assay Freundlich equation Glutaraldehyde 0210 nano-technology medicine.drug Nuclear chemistry |
| Zdroj: | Bulletin of Materials Science. 39:377-383 |
| ISSN: | 0973-7669 0250-4707 |
| DOI: | 10.1007/s12034-016-1161-7 |
| Popis: | The aim of this study was to develop a new variant of membranes based on collagen (COL), chitosan (CHI) and alkaline phosphatase (ALP) immobilized and cross-linking with glutaraldehyde (GA) at different concentrations. The biodegradation in the presence of collagenase was investigated. Biocompatibility was evaluated by MTT assay using a mouse fibroblast cell culture type NCTC (clone 929). Non-cross-linked samples were biocompatible and membranes cross-linked with low concentrations of GA (0.04, 0.08%) were also biocompatible. However, high concentrations of GA lead to a decreased biocompatibility. The adsorption behaviour of Ca2+ ions to all membranes were evaluated using the Freundlich isotherms. Haemolytic studies were performed in order to consider their applications in biomineralization process. By the addition of collagen and ALP to chitosan, the haemolytic index decreases, the COL–CHI–ALP membrane being in the non-haemolytic domain, while the COL–CHI–ALP–GA membrane has a haemolytic index greater than 2, and is slightly haemolytic. |
| Databáze: | OpenAIRE |
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