Investigation of Antibacterial Activity of Crude Extracts from Marine Snails and Bivalves in the Southern Coast of Vietnam
| Autor: | Pham Xuan Ky, Dao Viet Ha, Phan Bao Vy, Nguyen Phuong Anh, Pham Thi Mien, Le Ho Khanh Hy, Doan Thi Thiet |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
food.ingredient
Trochus maculatus biology 010405 organic chemistry Chemistry General Engineering Bacillus subtilis Snail biology.organism_classification 01 natural sciences 0104 chemical sciences Tectus pyramis food biology.animal parasitic diseases Serratia marcescens Botany Agar Antibacterial activity Tectus conus |
| Zdroj: | American Journal of Biomedical and Life Sciences. 7:10 |
| ISSN: | 2330-8818 |
| DOI: | 10.11648/j.ajbls.20190701.13 |
| Popis: | The primary antibacterial activity of methanol and chloroform crude extracts from marine snails and bivalves was assessed by using the agar diffusion technique against four bacterial strains. Active methanol extracts were then characterized using TLC, SDS-PAGE and FTIR. Methanol extracts from 5 snail species and 8 extracts from 12 bivalve species possessed the ability to inhibit Bacillus subtilis. Methanol extracts from 3 snail species Tectus conus, Maninella alounia and Trochus maculatus inhibited Escheria coli and those from 4 snail species Cerithium chinatum, Maninella alounia, Tectus pyramis, Trochus maculatus and the bivalve species Pinna bicolor exhibited activity against Serratia marcescens. Chloroform extracts from 7 snail species and those from 7 bivalve species showed inhibition on Bacillus subtilis. Only chloroform extract from the bivalve Chama cf dunkeri was active on Salmonella typhimur and that from the snail Trochus maculatus and bivalve Lopha cristagali inhibited Escheria coli. TLC and FTIR analysis of active methanol extracts showed the presence of amino acids, peptides and proteins. SDS-PAGE of those extracts also revealed proteins with a molecular weight range between 10 and 28 kDa. The obtained results indicate the potential antimicrobial compounds that could be explored in snail and bivalve in Vietnam. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|