Effect of specific nucleotide G 11 on cleavage activity of hairpin ribozymes in vitro *

Autor:	Bin Li, Youxin Jin, Jusheng Lin, Yu-Hu Song, Xin-Juan Kong, Nan-Zhi Liu
Rok vydání:	2003
Předmět:	Small hairpin RNA GlmS glucosamine-6-phosphate activated ribozyme biology GIR1 branching ribozyme Chemistry Ribozyme biology.protein General Materials Science Mammalian CPEB3 ribozyme Stem-loop Hairpin ribozyme Molecular biology VS ribozyme
Zdroj:	Progress in Natural Science. 13:345-349
ISSN:	1745-5391 1002-0071
DOI:	10.1080/10020070312331343650
Popis:	To study the effect of specific G11 nucleotide on cleavage activity of hairpin ribozyme in vitro, the 32P-labeled pKC transcript (267 nt) containing hepatitis B virus core region is used as target RNA. Synthetic hairpin ribozyme genes with G 11 mutational site are cloned into ribozyme vector pl.5 to create pHpRz for preparation of ribozyme. Non-labeled HpRz transcripts are incubated with 32P-labeled target-RNAs under different conditions and autoradiographed after gel electrophoresis. The results show that the cleavage activity of hairpin ribozyme is closely related to the matching of the bases between hairpin ribozyme and the substrate. The G11 site is not essential to cleavage activity of hairpin ribozyme in vitro, so the selection of target sequence matching G11 site of hairpin ribozyme is not limited to this site.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::6595b7ea2d9b3a18f4701257d6796f5a https://doi.org/10.1080/10020070312331343650 Zobrazit plný text záznamu