Photochemically-Generated Silver Chloride Nanoparticles Stabilized by a Peptide Inhibitor of Cell Division and Its Antimicrobial Properties

Autor: Felipe Nogueira Ambrosio, Dnane V. Almeida, Christiane Bertachini Lombello, Cesar de la Fuente-Nunez, Wanius Garcia, Geomar F. Cruz, Vani Xavier Oliveira, Marcelo D. T. Torres, Iris de Araujo, Fernanda Dias da Silva
Rok vydání: 2020
Předmět:
Zdroj: Journal of Inorganic and Organometallic Polymers and Materials. 30:2464-2474
ISSN: 1574-1451
1574-1443
DOI: 10.1007/s10904-019-01427-2
Popis: The 40 amino acid residues peptide MciZ (Mother Cell Inhibitor of FtsZ) represents a promising antibacterial agent as it is an effective inhibitor of bacterial cell division, Z-ring formation and localization. However, its efficacy is limited to Gram-positive bacteria as MciZ is unable to penetrate into Gram-negative organisms. In this study, we report the synthesis of plasmonic silver chloride nanoparticles stabilized by MciZ (NP/MciZ). NP/MciZ were synthesized using a fast and green route under blue light irradiation. Electron microscopy showed that NP/MciZ were enveloped by a peptide layer responsible for colloidal stability. X-ray diffraction analysis showed that silver chloride nanoparticles were crystalline in nature with small proportion of metallic silver. NP/MciZ showed a dose-dependent cytotoxicity against mammalian VERO cells. However, NP/MciZ exhibited remarkable antibacterial activity against Gram-positive bacterium B. megaterium comparable to MciZ. Furthermore, NP/MciZ showed similar antimicrobial activity against the Gram-negative bacterium E. coli and the yeast C. albicans. The photochemically-generated NP/MciZ presented here is a new organic–inorganic hybrid nanomaterial and has potential for biomedical or other applications.
Databáze: OpenAIRE
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