| Autor: |
Saitoh Hisato, Okada Saya |
| Rok vydání: |
2022 |
| Předmět: |
|
| Zdroj: |
International Journal of Membrane Science and Technology. 9:13-19 |
| ISSN: |
2410-1869 |
| DOI: |
10.15379/2410-1869.2022.09.01.02 |
| Popis: |
Although giant membrane vesicles prepared from paraformaldehyde-treated mammalian cells have been used to elucidate lipid and protein dynamics on the cell surface, there are few studies that have used them as a tool to deliver drugs to cells. Here we found that anti-cancer drug doxorubicin (Dox) was efficiently incorporated into and stably retained in giant membrane vesicles prepared from HeLa human cervical cancer cells. Intriguingly, coincubation of Dox-enclosed giant membrane vesicles with human pancreatic cancer-derived PK-59 and gastric cancer-derived KE-39 cells led to cell death. Microscopic observation of vesicle-docked cells revealed that docking of at least one to a few Dox-enclosed giant vesicles on the cellular cortex was sufficient to induce cell death, suggesting applicability of cell-derived giant membrane vesicles as an efficient drug delivery vector in cultured cell systems. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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