Efficient production of androstenedione by repeated batch fermentation in waste cooking oil media through regulating NAD+/NADH ratio and strengthening cell vitality of Mycobacterium neoaurum
| Autor: | Xia Menglei, Yanbing Shen, Zhihua Shang, Shuangping Xu, Min Wang, Zhou Xiuling, Xiao Zhang, Zhang Yang |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
Environmental Engineering biology Nicotinamide Renewable Energy Sustainability and the Environment Chemistry Phytosterol NADH dehydrogenase Bioengineering General Medicine 010501 environmental sciences biology.organism_classification 01 natural sciences Cofactor chemistry.chemical_compound Biotransformation Mycobacterium neoaurum 010608 biotechnology biology.protein Fermentation NAD+ kinase Food science Waste Management and Disposal 0105 earth and related environmental sciences |
| Zdroj: | Bioresource Technology. 279:209-217 |
| ISSN: | 0960-8524 |
| DOI: | 10.1016/j.biortech.2019.01.144 |
| Popis: | The bioprocess for producing androstenedione (AD) from phytosterols by using Mycobacterium neoaurum is hindered by nicotinamide adenine dinucleotides (NAD+ and NADH) ratio imbalance, insoluble substrate, and lengthy biotransformation period. This study aims to improve the efficiency of AD production through a combined application of cofactor, solvent, and fermentation engineering technologies. Through the enhanced type II NADH dehydrogenase (NDH-II), the NAD+/NADH ratio and ATP levels increased; the release of reactive oxygen species decreased by 42.32%, and the cell viability improved by 54.17%. In surfactant-waste cooking oil-water media, the conversion of phytosterol increased from 23.92% to 94.98%. Repeated batch culture successfully reduced the biotransformation period from 30 to 17 days, the productivity was 13.75 times more than the parent strain. This study is the first to improve the productivity of AD by enhancing NDH-II and provides a new strategy to increase the accumulation of NAD+-dependent metabolites during biotransformation. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect