Investigation of the physiochemical properties, cryoprotective activity and possible action mechanisms of sericin peptides derived from membrane separation
| Autor: | Xue-qing Geng, Yan Wu, Shaoyun Wang, Jinhong Wu, Zhang Li, Jianhua Liu, Zhengwu Wang, Ling Li, Xu Chen |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
chemistry.chemical_classification Chromatography biology Ice crystals Chemistry Scanning electron microscope Cell 04 agricultural and veterinary sciences biology.organism_classification 040401 food science Sericin Membrane technology Amino acid 03 medical and health sciences 030104 developmental biology 0404 agricultural biotechnology Membrane medicine.anatomical_structure Lactobacillus medicine Food Science |
| Zdroj: | LWT. 77:532-541 |
| ISSN: | 0023-6438 |
| DOI: | 10.1016/j.lwt.2016.12.004 |
| Popis: | In order to exploit their industrial applications, sericin peptides (3K-SP) were obtained by membrane separation and their physiochemical properties and cryoprotective function were investigated. Results showed that 3K-SP were mostly distributed less than 3000 Da, and rich in the amino acids Ser, Asp, Gly, Thr and Glu, which have been associated with the cryoprotective activity of ice-structuring proteins. Addition of 3K-SP to a frozen solution led to the reductions in melting temperature and melting time compared to control solution. In addition, 3K-SP inhibited ice recrystallization, since it could maintain small ice crystal sizes within a frozen solution. Furthermore, 3K-SP demonstrated high cryogenic protection activity of Lactobacillus delbrueckii Subsp. Bulgaricus during freezing, and provided optimal protection of cells at conditions in which its concentration was 1.0 mg/mL and the pH of the solution was 7.0. In these conditions, the percentage of surviving cells was as high as 84.78 ± 3.07%. Flow cytometric and scanning electron microscopy analyses showed that treatment with 3K-SP increased the percentage of viable cells from 52.9% to 80.1%, and suggest that 3K-SP may mediate its protective effects through interaction with cell membranes, whereby it surrounds cells in a glassy matrix that helps maintain their membrane integrity. |
| Databáze: | OpenAIRE |
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