Agrobacterium rhizogenes-mediated induction of adventitious rooting fromPinus contorta hypocotyls and the effect of 5-azacytidine on transgene activity

Autor: Anders Lindroth, Helena Franzen, David E. Clapham, Roland Grönroos, Haile S. Yibrah, Sara von Arnold
Rok vydání: 1996
Předmět:
Zdroj: Transgenic Research. 5:75-85
ISSN: 1573-9368
0962-8819
DOI: 10.1007/bf01969425
Popis: Bipartite constructs ofAgrobacterium rhizogenes strain LBA 9402 or A4RSII induced transformed roots on the hypocotyls ofPinus contorta following inoculation, LBA 9402 being more effective. The developmental sequence of root formation and morphology following infection were studied. Furthermore, the pattern of gene expression was studied during rooting and in roots using theuidA reporter gene driven by the 35S promoter. Morphologically most of the roots were normal, whether or not they expressed the reporter gene, but extensive proliferation of lateral roots was observed in some roots with β-glucuronidase (GUS) activity. All roots originated from tissues inside the endodermis, often similar to auxin-induced rooting in hypocotyl cutting as described by Gronroos and von Arnold (1987). Where the origin of GUS-positive roots could be traced, they developed from callus forming inside the endodermis. GUS activity was often observed along the root inside the endodermis, at the base of the lateral roots and at the root apex, but not in a region behind the apex. Stable integration of the transgene was verified using Southern blot analysis.
Databáze: OpenAIRE
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