Hog Kidney and Intestine Aminoacylase-Catalyzed Acylation of L-Methionine in Aqueous Media

Autor:	Anne Ferjancic-Biagini, Thierry Giardina, Antoine Puigserver, Max Reynier
Rok vydání:	1997
Předmět:	chemistry.chemical_classification Aminoacylase Methionine Biochemistry Catalysis Amino acid Acylation chemistry.chemical_compound Enzyme chemistry In vivo Yield (chemistry) Organic chemistry Biotechnology
Zdroj:	Biocatalysis and Biotransformation. 15:313-323
ISSN:	1029-2446 1024-2422
DOI:	10.3109/10242429709003197
Popis:	Synthesis of N-acyl-L-methionine derivatives was catalyzed by hog kidney and intestinal amino acylase in aqueous media using various acyl donors with acyl-chain lengths ranging from 1 to 6. Various reaction conditions were tested by varying the pH of the buffer, the water-miscible organic solvent and Zn2+ concentrations. Using short chain fatty acids, N-acyl-L-methionine derivatives were efficiently synthesized by both enzymes with a yield of about 20–30%, depending on the acid used. The optimal yield was achieved after a 17-h incubation of the reaction mixture containing 0.02 M acyl sodium salt, 0.2 M L-methionine, 10 μM ZnCl2 and 120 mU of aminoacylase in a 0.2 M sodium phosphate buffer at pH 6.0 and 37°C. The part potentially played by acylase in the in vivo acylation of amino acids, and by extension that of proteins in general, is discussed.
Databáze:	OpenAIRE
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