Synthesis and surface properties of new semi-fluorinated sulfobetaines potentially usable for 2D-electrophoresis
| Autor: | Mireille Starita-Geribaldi, Frédéric Guittard, Serge Geribaldi, Pascal Thebault, Elisabeth Taffin de Givenchy |
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| Rok vydání: | 2007 |
| Předmět: |
chemistry.chemical_classification
Gel electrophoresis Two-dimensional gel electrophoresis Chemistry Organic Chemistry Iodide Biochemistry Inorganic Chemistry Surface tension Hydrocarbon Pulmonary surfactant Critical micelle concentration Environmental Chemistry Organic chemistry Physical and Theoretical Chemistry Membrane fraction |
| Zdroj: | Journal of Fluorine Chemistry. 128:211-218 |
| ISSN: | 0022-1139 |
| DOI: | 10.1016/j.jfluchem.2006.12.008 |
| Popis: | New semi-fluorinated amidosulfobetaines, homologs of hydrocarbon amidosulfobetaines (ASB) commonly used in two-dimensional gel electrophoresis (2DE), were prepared in three steps from 2-F-alkylethyl iodide or F-alkyl iodide. Their synthesis was described and their air–water interface properties were investigated and compared with their perhydrogenated counterpart properties. The influence of the relative lengths of the perfluorinated and hydocarbonated moieties was discussed. 2DE of a rat testicular membrane fraction was performed comparatively using one of these fluorinated sulfobetaines and its hydrocarbon homolog; these preliminary results showed the great potential of the semi-fluorinated sulfobetaines in proteomic analysis. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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