Synthesis of wobble pairing oligoribonucleotides

Autor:	Eiko Ohtsuka, Makoto Koizumi, Shigenori Iwai, Yasutoshi Kawase
Rok vydání:	1989
Předmět:	Oligonucleotide Chemistry Stereochemistry General Chemistry General Medicine Wobble base pair Circular dichroism spectra Pairing Drug Discovery medicine Oligoribonucleotides Thermal stability Inosine medicine.drug
Zdroj:	Chemical and Pharmaceutical Bulletin. 37:2313-2317
ISSN:	1347-5223 0009-2363
DOI:	10.1248/cpb.37.2313
Popis:	Hexaribonucleotides containing inosine at a pairing position adjacent to the other four major nucleosides (GGINCC, N=A, C, G, U) were synthesized in solution by the phosphotriester method using 2'-O-tetrahydrofuranyl protecting groups. 5'-O-Dimethoxytrityl-2'-O-tetrahydropyranylinosine 3'-O-(metyl-N, N-diisopropyl)phosphor-amidite was used for solid-phase syntheses of decaribonucleotides (CGNGAUCICG, N=A, C, G, U). Thermal stabilities and circular dichroism spectra for these self-complementary duplexes were measured. The stabilizing effects of wobble base pairs in these duplexes were estimated as IC>IA>IU>IG.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::6555b6991eacc04448bd021402aafceb https://doi.org/10.1248/cpb.37.2313 Zobrazit plný text záznamu