Inter-species cross-reactivity of the prothoracicotropic hormone of Manduca sexta and egg-development neurosecretory hormone of Aedes aegypti
| Autor: | Walter E. Bollenbacher, Eva J. Katahira, Morton S. Fuchs, LaVern R. Whisenton, Alexej B. Bořkovec, Thomas J. Kelly |
|---|---|
| Rok vydání: | 1986 |
| Předmět: |
Aedes
medicine.medical_specialty animal structures biology Physiology Sphingidae education fungi Aedes aegypti biology.organism_classification Prothoracic gland chemistry.chemical_compound Endocrinology chemistry Biochemistry Manduca sexta Insect Science Internal medicine medicine Prothoracicotropic hormone Manduca Ecdysone |
| Zdroj: | Journal of Insect Physiology. 32:757-762 |
| ISSN: | 0022-1910 |
| Popis: | The cross-reactivities of the big and small forms of prothoracicotropic hormone (PTTH) from pupal brains of Manduca sexta and egg-development neurosecretory hormone (EDNH) from heads of adult Aedes aegypti were examined for PTTH by the in vitro Manduca prothoracic gland assay and for EDNH by the in vitro and in vivo Aedes ovary assays. The synthesis of ecdysone by both larval and pupal prothoracic glands of Manduca was increased in a dose-dependent manner by crude extracts of Aedes aegypti heads, reaching a maximum of approx. 3- and 2-fold, respectively. Gel filtration chromatography of the Aedes head extract revealed a peak of EDNH activity with an apparent mol. wt of 11 kD. This partially purified EDNH did not possess prothoracicotropic activity in the in vitro prothoracic gland assay, nor did any other fractions from the gel filtration column. Similarly, partially purified big and small PTTH did not activate Aedes atropalpus ovaries to synthesize ecdysone in vitro , nor did they cause ovarian maturation in vivo . Thus, it appears that the structural differences between PTTH and EDNH are sufficient enough to prevent functional cross-reactivity. The apparent discrepancy in the results obtained with the crude and partially purified EDNH and PTTHs raises questions about the reliability of bioassays for screening the presence and cross-reactivity of peptide neurohormones in crude extracts. |
| Databáze: | OpenAIRE |
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