PCR-RFLP analysis of the ITS2 region to identify Japanese Lymantria species (Lepidoptera: Lymantriidae)
| Autor: | Makoto Arimoto, Ren Iwaizumi |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
0106 biological sciences
0301 basic medicine Biology biology.organism_classification 01 natural sciences Lepidoptera genitalia 010602 entomology 03 medical and health sciences 030104 developmental biology Insect Science Lymantria xylina parasitic diseases Botany Lymantria dispar Internal transcribed spacer Restriction fragment length polymorphism Ribosomal DNA Lymantria mathura Lymantria |
| Zdroj: | Applied Entomology and Zoology. 51:63-70 |
| ISSN: | 1347-605X 0003-6862 |
| DOI: | 10.1007/s13355-015-0371-6 |
| Popis: | In this study, a polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP)-based method for species identification was applied to eight Japanese Lymantria species, including the four “Asian gypsy moth” species [Lymantria dispar japonica (Motschulsky), Lymantria umbrosa (Butler), Lymantria albescens Hori and Umeno, and Lymantria postalba Inoue] and four other species [Lymantria xylina Swinhoe, Lymantria mathura Moore, Lymantria monacha (Linnaeus), and Lymantria lucescens (Butler)]. We sequenced the second internal transcribed spacer (ITS2) region of nuclear ribosomal DNA (rDNA) of one individual from each of the eight species. We analyzed the recognition sites of a restriction endonuclease (MnlI) to identify the Lymantria species by PCR-RFLP. We then applied PCR-RFLP analysis to 330 individuals from 54 populations of the 8 species. As a result, these species could be correctly identified with the exception of two species pairs (L. dispar japonica and L. umbrosa; L. albescens and L. postalba). These results suggest that this PCR-RFLP method based on the ITS2 region is useful for identifying Japanese Lymantria species. |
| Databáze: | OpenAIRE |
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