Lipidomics of Mesenchymal Stromal Cells: Understanding the Adaptation of Phospholipid Profile in Response to Pro-Inflammatory Cytokines
| Autor: | Liliana Curado, Bebiana C. Sousa, Ana S. P. Moreira, Francisco dos Santos, Ana M. F. Oliveira Campos, Brígida Antunes, Tânia Melo, Pedro Domingues, M. Rosário M. Domingues, Elisabete Maciel |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Phosphatidylethanolamine Physiology Clinical Biochemistry Mesenchymal stem cell Phospholipid Lipid metabolism Cell Biology Phosphatidylserine Biology Cell biology Proinflammatory cytokine 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology chemistry Lipidomics lipids (amino acids peptides and proteins) Sphingomyelin |
| Zdroj: | Journal of Cellular Physiology. 231:1024-1032 |
| ISSN: | 0021-9541 |
| DOI: | 10.1002/jcp.25191 |
| Popis: | Mesenchymal stromal cells (MSCs) present anti-inflammatory properties and are being used with great success as treatment for inflammatory and autoimmune diseases. In clinical applications MSCs are subjected to a strong pro-inflammatory environment, essential to their immunosuppressive action. Despite the wide clinical use of these cells, how MSCs exert their effect remains unclear. Several lipids are known to be involved in cell's signaling and modulation of cellular functions. The aim of this paper is to examine the variation in lipid profile of MSCs under pro-inflammatory environment, induced by the presence of tumor necrosis factor alpha (TNF-α) and interferon gamma (IFN-γ), using the most modern lipidomic approach. Major changes in lipid molecular profile of phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylserine (PS), lysoPC (LPC), and sphingomyelin (SM) classes were found. No changes were observed in the phosphatidylinositol (PI) profile. The levels of PC species with shorter fatty acids (FAs), mainly C16:0, decreased under pro-inflammatory stimuli. The level of PC(40:6) also decreased, which may be correlated with enhanced levels of LPC(18:0), which is known to be an anti-inflammatory LPC, observed in MSCs subjected to TNF-α and IFN-γ. Simultaneously, the relative amounts of PC(36:1) and PC(38:4) increased. TNF-α and IFN-γ also enhanced the levels of PE(40:6) and decreased the levels of PE(O-38:6). Higher expression of PS(36:1) and SM(34:0) along with a decrease in PS(38:6) levels were observed. These results indicate that lipid metabolism and signaling are modulated during MSCs activation, which suggests that lipids may be involved in MSCs functional and anti-inflammatory activities. |
| Databáze: | OpenAIRE |
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