Transductional mapping of nine linked chromosomal genes in Bacillus thuringiensis

Autor: Hans G. Boman, Ritva Landén, Agneta Heierson
Rok vydání: 1983
Předmět:
Zdroj: Molecular and General Genetics MGG. 192:118-123
ISSN: 1432-1874
0026-8925
DOI: 10.1007/bf00327656
Popis: We have previously described a phage (ϕ63) for generalized transduction in Bacillus thuringiensis and used it for mapping of four chromosomal antibiotic resistance markers, namely nalA-rifA-strA-spcA (Landen et al. 1981). From ϕ63 we have now isolated a host range mutant called ϕ64 which contains 52–56 megadalton of DNA. Phage ϕ64 was found to be a more efficient transducing vector than ϕ63. The host range of ϕ64 is wide, with good growth on subspecies gelechiae, kurstaki, galleriae, thuringiensis and thompsoni, restriction on some derivatives of finitimus and ostrinae and no growth on alesti, israelensis and aizawai. Using ϕ64 and a series of new mutants of subspecies gelechiae we have no added five new genes to the antibiotic resistance group described before. The gene order found was guaB-purB-metA-novA-(purA-nalA)-rifA-strA-spcA. Linkage was also demonstrated between hisA and lysA.
Databáze: OpenAIRE
Externí odkaz: