Plastid Development in Leaf Epidermis
| Autor: | Jacob P. Varkey, Mathew J. Nadakavukaren |
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| Rok vydání: | 1981 |
| Předmět: | |
| Zdroj: | Proceedings, annual meeting, Electron Microscopy Society of America. 39:618-619 |
| ISSN: | 2690-1315 0424-8201 |
| DOI: | 10.1017/s0424820100099519 |
| Popis: | Changes in the plastid ultrastructure during development of a leaf have been studied extensively. Most studies have been done on the development of chloroplasts in mesophyll cells. Chloroplasts are small and few in number or plastids never develop into chloroplasts in epidermal cells of leaves of many species. Not much is known about the development of plastids in the epidermal tissue. Sunflower was selected to study the plastid development in the epidermal tissue.Sunflower plants were grown from seeds in growth chambers maintained on a 14:10 light:dark cycle at a daytime temperature of 78 F and nighttime temperature of 70 F with 80% humidity. Samples for ultrastructural study were taken from primary leaves at different stages of development. Because the rate of leaf development can vary from plant to plant, samples were taken from the same primary leaves of selected seedlings. These samples fixed in glutaraldehyde followed by osmium tetroxide were processed for electron microscopy using standard procedures. |
| Databáze: | OpenAIRE |
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