| Autor: |
Petr Vrátný, J. Čoupek, S. Vozka, Z. Hostomská |
| Rok vydání: |
1983 |
| Předmět: |
|
| Zdroj: |
Journal of Chromatography A. 254:143-155 |
| ISSN: |
0021-9673 |
| DOI: |
10.1016/s0021-9673(01)88327-8 |
| Popis: |
Two different ways of accelerating analyses of glucose oligomers by reversed-phase chromatography and of improving their sensitivity are presented. A specially developed glass cartridge packed with a C 18 bonded silica of the Separon type was used for high speed separations of maltodextrins up to a degree of polymerization of 8 at ambient temperature in 5 min using water as eluent. The effect of temperature upon the separation of carbohydrates on a conventional reversed-phase column in terms of heats of transfer, capacity factors, selectivity and column efficiency was also considered. The capacity factors decrease markedly with increasing column temperature owing to relatively high values of the heat of transfer. The non-linear increase in the heat of transfer with the degree of polymerization leads also to some reduction in the column selectivity at increased temperatures. An increase in temperature accelerates the rate of interconversion between the α- and β-anomers of saccharides, thus eliminating unwanted double peaks. The combined effects of elevated temperature result in a considerable acceleration in the separation of maltose (10 min for up to a degree of polymerization of 10) and cellobiose oligomers (10 min for a degree of polymerization of 6 at 70-80°C), which means a 2-20 fold acceleration of their analyses with a substantial gain in sensitivity. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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