Selective and Sensitive Determination of Mercury(II) Ions in River and Sea Water by an Automatic HPLC System Combined with On-line Column Enrichment
| Autor: | Sachi Okada, Youichi Fujii, Susumu Ichinoki |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Detection limit
Chromatography Chemistry Calibration curve Clinical Biochemistry Analytical chemistry Pharmaceutical Science chemistry.chemical_element Reversed-phase chromatography Biochemistry High-performance liquid chromatography Analytical Chemistry Mercury (element) Seawater Sample preparation Solid phase extraction |
| Zdroj: | Journal of Liquid Chromatography & Related Technologies. 28:1751-1764 |
| ISSN: | 1520-572X 1082-6076 |
| DOI: | 10.1081/jlc-200060482 |
| Popis: | A selective and sensitive determination method for mercury (Hg) ions in river and sea water by reversed‐phase high performance liquid chromatography (HPLC) has been developed. A one milliliter water sample containing Hg(II) ions was put into a 4 mL sample tube. To the sample tube, 2 mL of 0.5 mol/L (M) tetrabutylammonium bromide (TBA‐Br) solution was added to form a neutral Hg compound. The Hg compound subsequently became pre‐concentrated in a mini‐column packed with 5 µm ODS. By switching the valve, the Hg compound was separated in an ODS analytical column, and detected with a photometric detector (255 nm). These processes occur automatically, except for the addition of water samples and 0.5 M TBA‐Br. The correlation coefficient of the calibration curves (metal concentration vs. peak area) obtained with 1 mL of mercury standard was more than 0.9995 over the range of 1 ng/mL (ppb) to 10 µg/mL (ppm). The detection limit of Hg ions in 1 mL solution was 0.5 ppb, which corresponded to 3 times the sta... |
| Databáze: | OpenAIRE |
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