Transcriptome profiling of Plasmodium vivax in Saimiri monkeys identifies potential ligands for invasion
| Autor: | Monica Paneru, Louis H. Miller, Sarah L. Anzick, Craig Martens, Karthigayan Gunalan, J. Patrick Mershon, John W. Barnwell, Roberto R. Moraes Barros, José M. C. Ribeiro, Kishore Kanakabandi, Ramoncito L. Caleon, Kimmo Virtaneva, Juliana M. Sá |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Multidisciplinary biology 030231 tropical medicine Plasmodium vivax RNA biology.organism_classification Virology 03 medical and health sciences 030104 developmental biology 0302 clinical medicine Antigen parasitic diseases Gene expression Parasite hosting Merozoite surface protein Receptor Gene |
| Zdroj: | Proceedings of the National Academy of Sciences. 116:7053-7061 |
| ISSN: | 1091-6490 0027-8424 |
| Popis: | Unlike the case in Asia and Latin America, Plasmodium vivax infections are rare in sub-Saharan Africa due to the absence of the Duffy blood group antigen (Duffy antigen), the only known erythrocyte receptor for the P. vivax merozoite invasion ligand, Duffy binding protein 1 (DBP1). However, P. vivax infections have been documented in Duffy-negative individuals throughout Africa, suggesting that P. vivax may use ligands other than DBP1 to invade Duffy-negative erythrocytes through other receptors. To identify potential P. vivax ligands, we compared parasite gene expression in Saimiri and Aotus monkey erythrocytes infected with P. vivax Salvador I (Sal I). DBP1 binds Aotus but does not bind to Saimiri erythrocytes; thus, P. vivax Sal I must invade Saimiri erythrocytes independent of DBP1. Comparing RNA sequencing (RNAseq) data for late-stage infections in Saimiri and Aotus erythrocytes when invasion ligands are expressed, we identified genes that belong to tryptophan-rich antigen and merozoite surface protein 3 (MSP3) families that were more abundantly expressed in Saimiri infections compared with Aotus infections. These genes may encode potential ligands responsible for P. vivax infections of Duffy-negative Africans. |
| Databáze: | OpenAIRE |
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