Hbc Alone Profile: Susceptibility versus Protection against Hepatitis B Virus could be associated with TNF Production
| Autor: | Barreto Jose Augusto, Araujo Patricia, Latinni Flavia, Gonçalves Giovanna, S Diaz Ricardo |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Hepatitis
Hepatitis B virus HBsAg medicine.diagnostic_test business.industry medicine.medical_treatment T cell virus diseases medicine.disease medicine.disease_cause Virology digestive system diseases Flow cytometry Cytokine medicine.anatomical_structure Immunology medicine Tumor necrosis factor alpha Cytotoxicity business |
| Zdroj: | Virology & Mycology. |
| ISSN: | 2161-0517 |
| DOI: | 10.4172/2161-0517.1000120 |
| Popis: | Background: Donors with occult HBV (Hepatitis B virus) infection, defined as those who lacked detectable HBsAg but whose exposure to HBV infection was indicated by a positive anti-HBc (HBc alone) profile and the presence of HBV DNA, are a potential source of HBV infection. The aim of this study was to evaluate HBcAg-specific T cell responses, NK cell activity and cytokine levels in blood donors with HBc alone profiles with and without detectable viral DNA. Methods: From January 2010 to December 2012, a total of 4,252 HBc alone donations were obtained. Of the 4,252 donors, 681 donors had spontaneous HBV clearance (Co/s >10,0 by chemiluminescent assay, undetectable HBV DNA and reactivity to anti-HBc in subsequent donations), 3,097 were classified as false-positive for anti-HBc (Co/ s 10,0 in chemiluminescent assay, detectable HBV DNA and reactivity to anti-HBc in subsequent donations) and 36 were OBI (anti-HBc positive and detectable HBV DNA). There were 500 healthy blood donors and 434 HBV carriers (HBsAg, anti-HBc positive and detectable HBV DNA). NK cells were tested for cytotoxicity against K562 cells, serum levels of specific cytokines (IL-8, IL-1, IL-10, IL-12, IL-6 and TNF) were assayed by flow cytometry and HBcAg-specific T cell responses were assessed by lymphoproliferation reported in stimulation index (SI) units. Results: The IL-8 and IL-12 serum levels increased significantly (p 22.0). TNF-α was correlated with HBcAg-specific T cell responses in spontaneous HBV resolvers. Conclusion: Our results suggest that TNF-α production may be associated with protection against hepatitis B virus through increased NK cell activity and HBcAg-specific T cell responses in HBc alone blood donors. |
| Databáze: | OpenAIRE |
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