Direct proteomic profiling of human urine and blood serum in an experiment with five-day dry immersion

Autor: I. V. Dobrokhotov, O. A. Valeeva, N. M. Novoselova, Marc-Antoine Custaud, N. A. Pakharukova, L. Kh. Pastushkova, Irina M. Larina
Rok vydání: 2015
Předmět:
Zdroj: Human Physiology. 41:732-737
ISSN: 1608-3164
0362-1197
Popis: Changes in the proteome of urine and blood serum obtained from 14 healthy humans (aged 21–29 years), medically approved for the experiment with dry immersion, have been studied. Urine and serum samples were fractionated and concentrated using MB–WCX and MB–HIC magnetic particles, respectively. Direct mass spectrometry profiling by MSLDI–TOF was carried out using a ClinProt robotic device (Bruker Daltonics). As a result, on average, 143 proteins peaks in urine samples have been identified. The most plastic fraction of the urine proteome has been identified by a high variation coefficient (double of technical variation) in 23.7% of protein peaks. In blood serum, 175 peaks per sample have been identified, on average. Comparison of immersion mass spectra of the blood proteome and baseline revealed significant differences. It is concluded that the identified increase in peak areas for several protein fragments, including fragments of the C3 and C4 serum complement components and high-molecular-weight kinogen and fibrinogen, can be ascribed to human body adaptation to the experimental conditions.
Databáze: OpenAIRE
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