| Autor: |
Elsje Sigarlakie, Heinz Bauer |
| Rok vydání: |
1973 |
| Předmět: |
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| Zdroj: |
Journal of Microscopy. 99:205-218 |
| ISSN: |
0022-2720 |
| DOI: |
10.1111/j.1365-2818.1973.tb04673.x |
| Popis: |
SUMMARY Ultrathin frozen sections of glutaraldehyde fixed yeast cells have been successfully used for the demonstration of acid and alkaline phosphatase. Acid phosphatase was localized over the cell wall of both the mother cell and the bud as well as over the newly forming cross wall (septum). Cytoplasmic vesicles (vacuoles, lysosomes?) located close to the cell wall showed a positive reaction for acid phosphatase as well. After 3 h glutaraldehyde fixation an activation of the nuclear acid phosphatase was observed. Lead precipitates were predominantly found over the nucleolar material of ‘resting’ and budding cells. Alkaline phosphatase could be demonstrated in the ‘yeast-mesosome’ and within the plasmalemma invaginations. After separation of the bud, small vesicles, probably derived from the endoplasmic reticulum showed a strong positive reaction for alkaline phosphatase. In frozen sections incubated for alkaline phosphatase, non-specific lead precipitates were found in the nucleus and along the plasmalemma invaginations. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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