A fixture for X-ray crystallographic studies of biomolecules under high gas pressure

Autor:	R. F. Tilton
Rok vydání:	1988
Předmět:	chemistry.chemical_classification Diffraction Materials science Biomolecule Resolution (electron density) X-ray chemistry.chemical_element Fixture General Biochemistry Genetics and Molecular Biology Crystal Crystallography chemistry Beryllium Powder diffraction
Zdroj:	Journal of Applied Crystallography. 21:4-10
ISSN:	0021-8898
DOI:	10.1107/s0021889887008173
Popis:	A fixture is described for collecting X-ray diffraction data from biomolecule crystals under gas pressures up to 300 atm (30 MPa). The fixture is completely portable and can be used on conventional diffractometers. Normal procedures for crystal mounting, alignment and data collection require only slight modification. A test data set of the protein sperm whale metmyoglobin under a gas pressure of 145 atm of N2 gas has been collected to a nominal resolution of 2.0 Å using Cu Kα radiation and refined to an R-factor of 16.2%. Data collection beyond 2.0 Å resolution is hampered by the presence of strong powder diffraction from the beryllium shroud.
Databáze:	OpenAIRE
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