QUANTITATIVE HPLC ANALYSIS OF 4-[4-4-(CHLOROPHENYL)PHENYL]-4-OXO-2S-(PHENYLTHIOMETHYL) BUTANOIC ACID (BAY 12-9566), A METALLOPROTEINASE INHIBITOR, AND ITS METABOLITES IN HUMAN PLASMA
| Autor: | G. J. Krol, D. L. Rose, V. K. Agarwal |
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| Rok vydání: | 1999 |
| Předmět: |
Detection limit
Chromatography Elution Metabolite Clinical Biochemistry Pharmaceutical Science Reversed-phase chromatography Biochemistry High-performance liquid chromatography Analytical Chemistry chemistry.chemical_compound chemistry Sample preparation Quantitation Range Quantitative analysis (chemistry) |
| Zdroj: | Journal of Liquid Chromatography & Related Technologies. 22:1893-1906 |
| ISSN: | 1520-572X 1082-6076 |
| Popis: | A High Performance Liquid Chromatographic (HPLC) method was developed for the analysis of Bay 12-9566 (4-[4-4-(chlorophenyl)Phenyl]-4-oxo-2S-(phenylthiomethyl) butanoic acid), a metalloproteinase inhibitor, and its three metabolites, M1 (sulfoxide), M2 (ρ-hydroxy) and M3 (reduction product) in plasma. Sample preparation involved precipitation of plasma proteins using acidified acetonitrile. A reverse phase chromatography with gradient elution, ultraviolet detection at 290 nm and internal standard were used for separation and quantitation of all analytes. The quantitation range was 0.10 μg/mL to 50 μg/mL for BAY 12-9566, and 0.10 μg/mL to 3.00 μg/mL for all three metabolites. The limit of detection for BAY 12-9566 and for all three metabolites was 0.05 μg/mL. Intra-day accuracy for BAY 12-9566 and the three metabolites ranged from 95.0 to 105.0% and precision (CV) ranged from 0.0 to 4.76%. Inter-day accuracy and precision were based on quality control samples analyzed concurrently with subject plasma sampl... |
| Databáze: | OpenAIRE |
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