Parametric analysis of length distributions of the fission yeast, Schizosaccharomyces pombe, in chemostat culture
| Autor: | Hisao Miyata, Byron F. Johnson, Machiko Miyata |
|---|---|
| Rok vydání: | 1995 |
| Předmět: | |
| Zdroj: | The Journal of General and Applied Microbiology. 41:63-73 |
| ISSN: | 1349-8037 0022-1260 |
| DOI: | 10.2323/jgam.41.63 |
| Popis: | Assuming that cells grow exponentially in length, the cycle of cells in chemostat cultures was analyzed parametrically by the age-size method (James, T. W. et al., Exp. Cell Res., 94, 267-276, 1975), which fitted well to the raw data of the length distribution. For simulation, we used the cell length and coefficient of variation at division from empirical data. The cell length and coefficient of variation at birth, the specific growth rate in extension, and the proportion of growing cells were estimated for simulation. We found that the stop-grow point of a cycle depended on the culture conditions: cells grown at D = 0.247 h −1 in the chemostat culture had the stop-grow point at 0.72 of the cycle, similar to cells growing exponentially in the batch culture. Cells grown at D = 0.126 h −1 and D = 0.062 h −1 in the chemostat cultures had the stop-grow points at 0.85 and 0.97 of the cycles, respectively. There was a sub-population of nongrowing cells estimated |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|