Effect of feeding strategies on inulinase production analyzed in a biocalorimeter
| Autor: | S. Ashwath Balaje, N. E. Sivanesh, Perinkulam Ravi Deepa, Santharam Leelaram, Mahadevan Surianarayanan |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine Inulinase activity Sucrose biology Bioenergetics Chemistry Bioengineering biology.organism_classification Metabolic heat 01 natural sciences Applied Microbiology and Biotechnology Biochemistry 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology Kluyveromyces marxianus Reaction calorimeter 010608 biotechnology Batch processing Food science Inulinase |
| Zdroj: | Process Biochemistry. 51:692-703 |
| ISSN: | 1359-5113 |
| DOI: | 10.1016/j.procbio.2016.03.012 |
| Popis: | Kluyveromyces marxianus exoinulinase production in shaker and bio reaction calorimeter has been studied. Biocalorimetric studies revealed enhanced inulinase production due to the linear and exponential feeding of sucrose. Linear fed-batch inulinase production (355 IU/mL) was seventeen-fold greater than batch mode (20.98 IU/mL). Further controlling the specific growth rate by exponential feeding of sucrose gave highest inulinase activity of 728 IU/mL. Exponential feeding strategy yielded highest inulinase activity in the synthetic medium with a reduction in batch time to half that of the linear feeding. This has been substantiated by metabolic and physiological aspects of inulinase production. A Michaelis-Menten type empirical model was developed for fed-batch that can be adopted for controlling specific growth rate based on metabolic heat responses, towards achieving maximum enzyme production and release. |
| Databáze: | OpenAIRE |
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