The Effect of B9 and B12 Vitamins Deficiency on Hypomethylation of MMP-9 gene Promoter Among Women With Preterm Parturition

Autor: Abotorabi Sh, Habibi Nozari F, Moeini N, Momeni Am, Sahar Moghbelinejad, Zargar M
Rok vydání: 2021
Předmět:
Zdroj: Biochemical Genetics. 60:336-350
ISSN: 1573-4927
0006-2928
DOI: 10.1007/s10528-021-10099-y
Popis: Preterm birth is one of the problems that pregnant women may encounter during pregnancy. Over-expression of Matrix metalloproteinases (MMPs) has the main role in the untimely remodeling of the extracellular matrix (ECM) and preterm delivery, but its exact mechanism is unknown. This study was undertaken to examine the methylation and expression patterns of this gene in women delivering preterm and also to analyze the correlation of serum B9 and B12 vitamins levels with expression and methylation of MMP-9 gene. The MMP-9 RNA and protein levels in 50 women delivering preterm and 50 women delivering at term were determined by Real-time PCR and enzyme-linked immunosorbent assay (ELISA) methods. Methylation pattern of this gene was evaluated by Epitect Methyl-II PCR assay and methylation-sensitive restriction enzymes combined with PCR amplification method; then, their correlation with serum B9 and B12 vitamins was studied. Placental MMP-9 RNA and protein levels were higher (P
Databáze: OpenAIRE
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