Autoradiolysis of the 2-deoxy-2-[18F]fluoro-D-glucose radiopharmaceutical
| Autor: | E. Búriová, F. Melichar, F. Macášek, L. Procházka, M. Kropacek |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Arabinose
Electrospray Chromatography Health Toxicology and Mutagenesis Public Health Environmental and Occupational Health Pollution High-performance liquid chromatography Thin-layer chromatography Analytical Chemistry chemistry.chemical_compound Nuclear Energy and Engineering chemistry D-Glucose Yield (chemistry) Radiolysis Radiology Nuclear Medicine and imaging Fluoride Spectroscopy |
| Zdroj: | Journal of Radioanalytical and Nuclear Chemistry. 264:595-602 |
| ISSN: | 1588-2780 0236-5731 |
| DOI: | 10.1007/s10967-005-0759-9 |
| Popis: | To control virtually the toxic compounds and to improve quality control of the solution of 2-deoxy-2-[18F]fluoro-d-glucose (2-[18F]FDG), the products of its autoradiolysis were analyzed by high-performance liquid chromatography with electrospray mass spectrometric and radiometric detectors (HPLC/MS/RAD), thin layer chromatography on TLC silica plate and HPTLC on amino modified silica plate. Except Kryptofix™ 2.2.2, glucose and fluoride anion, no by-products and impurities were observed by LC/MS analysis of fresh 2-[18F]FDG samples. The analysis performed in the time interval of 6 to 48 hours after the end of 2-[18F]FDG synthesis indicated that the activity of the autoradiolysis products separated by HPLC did not exceed 1.3%. As the main autoradiolysis products of 3.3 . 10-5 to 4.4 . 10-5M 2-[18F]FDG solution of original specific activity 0.5-1.5 GBq . cm-3 were established: arabinose - 2.8 μM (G= 0.07/100 eV), gluconic and glucuronic acids 1.8-0.5 μM (G =0.01-0.05/100 eV), arabinose and araburonic acids occurred under 0.5 μM concentration at residual glucose contents about 0.14 mM. Radiation chemical yields of active products were calculated from molar activity of 2-[18F]FDG and the percentage of their activity: 0.5% radiochemical yield of 2-[18F]fluoroglucuronic acid corresponds to the G = 0.004/100 eV and 0.3% yield of 2-[18F]fluorogluconic acid issues G = 0.003/100 eV. |
| Databáze: | OpenAIRE |
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