| Autor: |
M.D. Chiflikian, A. R. Armenian, H. Ch. Buniatian |
| Rok vydání: |
1980 |
| Předmět: |
|
| DOI: |
10.1016/b978-0-08-025921-5.50019-9 |
| Popis: |
This chapter discusses a study to examine the effect of GABA on spontaneous and evoked release of 3 H-NA from synaptosomes of rat brain mesodiencephalic region. GABA in concentration 10 −4 and 10 –3 M has been shown to increase the spontaneous release of H-NA from synaptosomee of mesodlencephallc region. This effect appeared immediately after the addition of GABA and lasted about 1 min. The effect of GABA was blocked by 3 × 10 −6 M and 10 −5 M bicuculline that itself stimulates the spontaneous efflux of 3 H-NA and this effect developed gradually. GABA significantly inhibited electrically evoked release of 3 H-NA from synaptosomes. In the presence of bicuculline, the effect of GABA was not altered, whereas bicuculline as a sole substrate reduced the electrically evoked release of 3 H-NA. GABA blocked the K + -evoked (40 mM) and protoveratrine-evoked (5 μM) release of 3 H-NA from synaptosomes. In the Ca-free medium, the effect of GABA on the K + -evoked release of 3 H-NA was reversed. The results obtained suggest the role of GABA in the presynaptic regulation of NA release in brain. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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