Identification of somaclonal variants of sugarcane (Saccharum spp.) resistant to sugarcane mosaic virus via RAPD markers

Autor:	Maira Oropeza, Palmira Guevara, José Luis Ramírez, Eva de García
Rok vydání:	1995
Předmět:	Genetics biology Sugarcane mosaic virus Somatic embryogenesis Plant virus Callus Potyvirus Plant Science Plant disease resistance biology.organism_classification Molecular Biology RAPD Somaclonal variation
Zdroj:	Plant Molecular Biology Reporter. 13:182-191
ISSN:	1572-9818 0735-9640
Popis:	Somaclonal variants resistant to sugarcane mosaic virus (SCMV) were obtained from susceptible sugarcane cv PR62258 through somatic embryogenesis by increasing the number of subcultures of the embryogenic callus tissue in MS medium with 3 mg/L 2,4-dichlorophenoxyacetic acid. Transfers were made at 30-day intervals for 1, 2 or 3 subcultures. Two somaclones, namely AT626 and BT627, were selected by their resistance to SCMV. These subclones have maintained the resistance trait over seven years of testing in the field. In this report we identified the somaclonal SCMV resistant variants from the maternal line and the nonresistant somaclones, using the RAPD technique.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::64b194375f49f000f12afacc5b45b3f9 https://doi.org/10.1007/bf02668790 Zobrazit plný text záznamu Plný text ve formátu PDF