| Autor: |
David Kritchevsky, Janet S. Carlton, Himanshu V. Kothari |
| Rok vydání: |
1973 |
| Předmět: |
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| Zdroj: |
Journal of Labelled Compounds. 9:365-371 |
| ISSN: |
0022-2135 |
| DOI: |
10.1002/jlcr.2590090221 |
| Popis: |
A simplified procedure is described for the synthesis and purification of labelled cholesteryl esters. The esters are synthesized enzymatically from (4-14C) cholesterol and free fatty acids. The separation of the lipids from aqueous medium is simplified by lyophilizing the reaction mixture at equilibrium. The lipid components of the freeze-dried powder are separated by silicic acid chromatography. The radiochemical purity of the product was shown to be greater than 98%. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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