Studies of the mixing character and flow distribution in mycelial fermentation broths
| Autor: | J. J. Vanags, M. A. Priede, U. E. Viesturs |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
biology
Aspergillus niger Mixing (process engineering) Pellets Bioengineering Industrial fermentation biology.organism_classification Applied Microbiology and Biotechnology chemistry.chemical_compound Chemical engineering chemistry Botany Bioreactor Fusicoccum amygdali Fermentation Citric acid Biotechnology |
| Zdroj: | Acta Biotechnologica. 15:355-366 |
| ISSN: | 1521-3846 0138-4988 |
| Popis: | The influence of two mixing systems on the principal parameters of mycelial fermentations of Aspergillus niger, Fusicoccum amygdali Del. and Fusarium moniliforme Sheld. as well as their metabolite citric acid, fusicoccin and gibberellic acid production was analyzed from the viewpoint of flow energy distribution in a bioreactor. The growth and metabolite synthesis during fermentation was compared under different mixing conditions in the fermenter FU-8 with a turbine mixing system (TMS) and a counterflow mixing system (CMS). It was found that the growth, productivity and respiration characteristics as well as the morphology of these cultures varied dependent on the mixing system and agitation regime used. The counterflow mixing system was more favourable for large agglomerates (F. amygdali) or soft pellets (A. niger) forming fungi, while the turbine mixing system was more effective for F. moniliforme growing in the form of small clumps and freely dispersed hyphae. Flow characteristics under different mixing conditions were analyzed in a model fermenter. The kinetic energy of flow fluctuations was measured in gassed and ungassed water and different fermentation broth systems by using a Stirring Intensity Measuring Device (SIMD-F1). The difference of the energy values at different points was better expressed in the fermenter with a turbine mixing system in comparison with that having a counterflow mixing system. High viscous F. amygdali and A. niger broth provided higher energy values compared to water and low viscous F. moniliforme broth. It was observed that the intensity of growth and the intensity of the synthesis decreased at very high energy values, which was obviously connected to the influence of the irreversible shear stress on the mycelial morphology. |
| Databáze: | OpenAIRE |
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