Cytophysiological Features of the Cereal-Based Experimental System 'Embryo In Vivo–Callus In Vitro'

Autor: G E Titova, N. N. Kruglova, A.E. Zinatullina, O. A. Seldimirova
Rok vydání: 2021
Předmět:
Zdroj: Russian Journal of Developmental Biology. 52:199-214
ISSN: 1608-3326
1062-3604
Popis: The most important problem in studying plant calluses in vitro is the relationship between endogenous and exogenous factors that affect the formation of callus (“callus formation”) and their development in an induction medium (“callusogenesis”). Such an endogenous factor as the cytophysiological status of explants in vivo and of calluses in the dynamics of in vitro cultivation attracts the particular attention of the scientific community. In this review, considering the example of cereals, the literature and the authors’ own data on the identification of histological and hormonal features of initial callus cells in explants, immature embryos in vivo, and morphogenic calluses formed from them during in vitro development have been analyzed. The answers to some controversial questions related to the induction of morphogenetic competence and reprogramming of the development of initial callus cells presented in the literature are considered. The comparison of callus formation and callusogenesis in vitro with some similar events in vivo confirms the validity of the principle of universality of morphogenesis processes in vivo and in vitro (Batygina, 2014 and earlier). The prospects applying an integrated experimental system “embryo in vivo–callus in vitro” as a model for studying plant morphogenesis, one of the most complex biological phenomena, is discussed.
Databáze: OpenAIRE
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