Immunoaffinity Chromatography Clean-Up and LC for Analysis of Salinomycin and Narasin in Chicken Muscle

Autor: Cun Li, Jianzhong Shen, Sijun Zhao, Suxia Zhang, Xiaowei Li, Jiang Haiyang, Youn-Kyoung Ra
Rok vydání: 2008
Předmět:
Zdroj: Chromatographia. 68:701-706
ISSN: 1612-1112
0009-5893
DOI: 10.1365/s10337-008-0857-2
Popis: A method has been developed for simultaneous analysis of salinomycin and narasin in chicken muscle. Muscle samples were extracted with acetonitrile. Clean-up of the extracts on an immunoaffinity chromatography column was followed by liquid chromatography with postcolumn derivatisation and UV–visible detection at 520 nm. The immunoaffinity columns were prepared by coupling the anti-salinomycin monoclonal antibody to CNBr-activated Sepharose 4B. When chicken muscle fortified at 5, 25, and 50 ng g−1 was analyzed, intra-assay mean recoveries of salinomycin and narasin were in the ranges 87.5–93.1 and 86.2–94.3%, respectively, with relative standard deviation (RSD) of 4.7–6.2 and 2.4–5.7%. Inter-assay mean recoveries were 86.0–93.0 and 86.0–92.1%, respectively, with RSD of 4.8–6.5 and 5.8–7.4%. The limit of detection of the method was 2.5 ng g−1 for both drugs in chicken muscle.
Databáze: OpenAIRE
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