| Autor: |
Kriston Garrison, Erdogan Memili, Song-Yi Jung, Laura Greer, Jean M. Feugang, Christi Wolgemuth, Nelida Rodriguez-Osorio, Mark A Crenshaw, Hongfeng Wang |
| Rok vydání: |
2009 |
| Předmět: |
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| Zdroj: |
Livestock Science. 121:102-107 |
| ISSN: |
1871-1413 |
| DOI: |
10.1016/j.livsci.2008.06.013 |
| Popis: |
In vitro -production of porcine embryos is crucial for biomedical and agricultural research, however, current culture systems for porcine embryos are sub-optimal, and the developmental potential of in vitro -produced embryos is not well studied. The objectives of this study were to evaluate whether PZM-3 or NCSU-23 better support porcine embryo development and the effects of α-amanitin and cycloheximide on porcine embryonic development. Briefly, porcine presumptive zygotes were produced after in vitro maturation/fertilization (IVM/IVF) and cultured either in NCSU-23 or PZM-3 culture media. Transcript levels of BAX and BCL2L1 genes from blastocysts were detected by using Real-time PCR. The effects of α-amanitin and cycloheximide were evaluated for the role of inhibiting transcription and translation during early porcine embryogenesis. Results showed that both cleavage and blastocyst rates decreased significantly in NCSU-23 group compared as PZM-3 group. However, BAX and BCL2L1 transcript levels were similar in blastocysts cultured in both PZM-3 and NCSU-23 media. When porcine embryos cultured in PZM-3, cleavage rates were significantly decreased in the present of cycloheximide and both α-amanitin and cycloheximide treatments completely inhibited the blastocyst formation. Results showed that PZM-3 medium better supported porcine early embryonic development than NCSU-23 medium, and the inhibition of embryonic genome activation does not completely stop embryo cleavage but prevents development to the blastocyst stage. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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