| Autor: |
Marc Fellous, D. Crevat, A.M. Courouce, C. Ropars, Jorge Kalil, J. Drouet |
| Rok vydání: |
1981 |
| Předmět: |
|
| Zdroj: |
Annales de l'Institut Pasteur / Immunologie. 132:319-326 |
| ISSN: |
0769-2625 |
| DOI: |
10.1016/0769-2625(81)90080-5 |
| Popis: |
Two BALB/c mice were immunized 4 times with a mixture of adw2 and ayw4 subtypes of HBs antigens. Their spleens were then hybridized with mouse myeloma cell line NS1. Using three different radioimmuno-assays (RIA), 264 independent hybridomas were screened for anti-HBs activity. By at last one of these techniques, 95% of the colonies were positive. Selected colonies were cloned and supernatants studied by RIA and immunodiffusing, techniques for specificity characterisation. Some clones recognised the common ⪡ a ⪢ subtype, and other were directed to more restricted specificities. Ascites fluid was active on RIA up to 10 7 dilution (up to 29.000 Ul/ml). These monoclonal antibodies may be powerfull reagent for the diagnosis and understanding of viral hepatitis B. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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