Binding and penetration ofRhizobium japonicum to cultured soybean cells

Autor:	Shahnaz Malek-Hedayat, Siu-Cheong Ho
Rok vydání:	1987
Předmět:	chemistry.chemical_classification biology food and beverages Lectin Peptide Cell Biology Penetration (firestop) Biochemistry Molecular biology Agar plate chemistry.chemical_compound chemistry Cell culture Galactose biology.protein Antibody Soybean agglutinin Molecular Biology
Zdroj:	Glycoconjugate Journal. 4:391-398
ISSN:	1573-4986 0282-0080
DOI:	10.1007/bf01048372
Popis:	A cultured soybean cell line, SB-1 was used to evaluate the initial interaction between the soybean cells andRhizobium japonicum. Co-culturing ofR. japonicum with SB-1 cells in suspension resulted in strain-specific polar attachment. This attachment can be inhibited by galactose and antibodies raised against seed soybean agglutinin (SBA). A lectin was purified from SB-1 cells which shares properties with SBA in terms of immunological reactivity, sugar binding activity, polypeptide molecular weight and peptide maps. When the SB-1 cells were co-cultured withR. japonicum for three weeks in solid agar medium, histological staining revealed bacterial penetration into certain SB-1 cells. Furthermore, there were focal regions of cells with prominent nuclei representing actively proliferating regions. These observations are analogous to that ofin vivo nodule initiation in soybean roots.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::64612c3bbb576e96749103b55ab93a70 https://doi.org/10.1007/bf01048372 Zobrazit plný text záznamu Full text from SpringerLink