Rabbit Sertoli Cells: Immunohistochemical Profile from Neonatal to Adult Age
Autor: | Valeria Grieco, B. Banco |
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Rok vydání: | 2018 |
Předmět: |
Pathology
medicine.medical_specialty 030219 obstetrics & reproductive medicine biology 040301 veterinary sciences Cell Rabbit (nuclear engineering) 04 agricultural and veterinary sciences Sertoli cell Adult age 0403 veterinary science 03 medical and health sciences 0302 clinical medicine Immunophenotyping medicine.anatomical_structure Antigen biology.protein medicine Immunohistochemistry Antibody |
Zdroj: | Methods in Molecular Biology ISBN: 9781493976973 |
DOI: | 10.1007/978-1-4939-7698-0_4 |
Popis: | Immunohistochemistry (IHC) on formalin-fixed paraffin-embedded tissue specimens is a widely used laboratory technique that allows the demonstration and the direct microscopical observation of antigens within tissue sections by means of specific antibodies. IHC is one the best tools to define the immunophenotype of a cell during maturation or neoplastic transformation.To investigate the immunophenotype of postnatal rabbit Sertoli cell, from neonatal to adult age, in this chapter, the immunohistochemical protocol described is one of the most common: the avidin-biotin complex (ABC) method. |
Databáze: | OpenAIRE |
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