Determination of trimethylamine-N-oxide in combination withl -carnitine andγ-butyrobetaine in human plasma by UPLC/MS/MS
| Autor: | Maija Dambrova, Dace Hartmane, Edgars Liepinsh, Eduards Sevostjanovs, Ilze Konrade, Ieva Strele, Gustavs Latkovskis, Osvalds Pugovics, Solveiga Grinberga |
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| Rok vydání: | 2015 |
| Předmět: |
Pharmacology
Analyte Chromatography Hydrophilic interaction chromatography Clinical Biochemistry Trimethylamine N-oxide General Medicine Mass spectrometry Biochemistry High-performance liquid chromatography Analytical Chemistry chemistry.chemical_compound chemistry Human plasma Drug Discovery medicine Ammonium Carnitine Molecular Biology medicine.drug |
| Zdroj: | Biomedical Chromatography. 29:1670-1674 |
| ISSN: | 0269-3879 |
| Popis: | An ultra-high-performance liquid chromatography-mass spectrometry (UPLC/MS/MS) method was developed and validated for the quantification of trimethylamine-N-oxide (TMAO) simultaneously with TMAO-related molecules L-carnitine and γ-butyrobetaine (GBB) in human blood plasma. The separation of analytes was achieved using a Hydrophilic interaction liquid chromatography (HILIC)-type column with ammonium acetate-acetonitrile as the mobile phase. TMAO determination was validated according to valid US Food and Drug Administration guidelines. The developed method was successfully applied to plasma samples from healthy volunteers. |
| Databáze: | OpenAIRE |
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