| Autor: |
Carmina Villariba Tolentino, Ana Maria Cariño, Kin Israel Notarte, Imee Macaranas, Allan Fellizar, Rock Christian Tomas, Lara Mae Angeles, Lorenzo Abanilla, Antonio Lim, Ma. Kristina Carmela Aguilar, Pia Marie Albano |
| Rok vydání: |
2021 |
| DOI: |
10.21203/rs.3.rs-437863/v1 |
| Popis: |
Background: Some E. coli strains that synthesize the toxin colibactin within the 54-kb pks island are being implicated in colorectal cancer (CRC) development. Here, the prevalence of pks+ E. coli in malignant and benign colorectal tumors obtained from selected Filipino patients was compared to determine the association of pks+ E. coli with CRC in this population. Methods and Results: A realtime qPCR protocol was developed to quantify uidA, clbB, clbN, and clbA genes in formalin fixed paraffin embedded colorectal tissues. The number of malignant tumors (44/62; 71%) positive for the uidA gene was not significantly different (p=0.3428) from benign (38/62; 61%) tumors. Significantly higher number of benign samples (p<0.05) were positive for all three colibactin genes (clbB, clbN, and clbA) compared with malignant samples. There was also higher prevalence of pks+ E. coli among older females and in tissue samples taken from the rectum. Conclusion: Hence, pks+ E. coli may not be associated with CRC development among Filipinos. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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