Competency of human-derivedMycobacterium lepraeto use palmitic acid in the synthesis of phenolic glycolipid-I and phthiocerol dimycocerosate and to release C02in axenic culture

Autor: Frommel D, Harris Eb, Guebre-Xavier M, Haile-Mariam Hs, Shannon Ej
Rok vydání: 1992
Předmět:
Zdroj: Leprosy Review. 63
ISSN: 0305-7518
Popis: Insufficient numbers of viable Mycobacterium leprae have hampered metabolic studies using human-derived M. leprae. In this study, sufficient numbers of M. leprae were obtained from an untreated lepromatous patient to titrate the effects of pH on the metabolism of 14C-palmitic acid by M. leprae. Catabolic metabolism (oxidation of 14C-palmitic acid and release of 14CO2) was maximal when M. leprae were incubated at 33 degrees C and suspended in Middlebrook 7H9, ADC supplemented medium that had been buffered to maintain a pH of 4.8. Anabolic metabolism (synthesis of 14C-phenolic glycolipid-I and its precursor, 14C-phthiocerol dimycocerosate) was maximal when the pH was maintained at 6.8.
Databáze: OpenAIRE