Improved utilization of tissue blocks for electron microscopy

Autor:	M.E. Matthiessen, P. Rømert
Rok vydání:	1979
Předmět:	Pathology medicine.medical_specialty Histology Chromatography Tissue Preservation Liver cell Formaldehyde law.invention chemistry.chemical_compound chemistry law Ultrastructure medicine Glutaraldehyde Anatomy Electron microscope Fixative Fixation (histology)
Zdroj:	Cells Tissues Organs. 104:388-393
ISSN:	1422-6421 1422-6405
DOI:	10.1159/000145089
Popis:	Liver tissue from miniature pig fetuses was immersion-fixed in fixative mixtures with various concentrations of formaldehyde and glutaraldehyde. The preservation quality of hepatocytes was evaluated ultrastructurally in a peripheral zone (30–130 µm below the surface) and a central zone (500 µm below the surface). In the peripheral zone the best preservation was obtained with a fixative mixture containing 2% formaldehyde and 2% glutaraldehyde and in the central zone with a fixative mixture containing 8% formaldehyde and 8% glutaraldehyde. It is concluded that a better utilization of fairly large tissue blocks for ultrastructural investigation can be obtained by division of the block and subsequent fixation in fixatives containing various concentrations of formaldehyde and glutaraldehyde.
Databáze:	OpenAIRE
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