Validation of a DNA biochip for species identification in food forensic science
| Autor: | Valentina Campia, Paola Modesto, Maria Vittoria Riina, Maria Grazia Maniaci, Simone Peletto, Cristina Biolatti, Silvia Trisorio, Pier Luigi Acutis, Chiara Beltramo, Silvia Colussi |
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| Rok vydání: | 2017 |
| Předmět: |
Laboratory methods
Computer science business.industry 010401 analytical chemistry Pasteurization 04 agricultural and veterinary sciences 040401 food science 01 natural sciences Animal origin 0104 chemical sciences Biotechnology law.invention 0404 agricultural biotechnology law Species identification business Animal species Biochip Analysis method Food Science |
| Zdroj: | Food Control. 78:366-373 |
| ISSN: | 0956-7135 |
| Popis: | Authentication of food product ingredients by food control agencies relies on analytical laboratory methods for animal species identification. Such methods must be validated if their results are to be used as acceptable evidence in a court of law. Following the validation guidelines of the Scientific Working Group on DNA Analysis Methods (SWGDAM), we carried out a validation process for the Low Cost and Density (LCD) Array (MEAT 5.0 version) kit for food forensics based on a DNA biochip technology that simultaneously detects 24 animal species. Mixtures of the animal species the kit is designed to detect were prepared at different concentrations and tested on raw/pasteurized, and heated meat and milk matrices. The LCD Array showed high specificity and high sensitivity and it appeared to be robust and repeatable. As such, it provides a useful tool for detecting common adulterants in food items of animal origin. |
| Databáze: | OpenAIRE |
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