High-intensity focused ultrasound combined with herpes simplex virus thymidine kinase gene-loaded ultrasound-targeted microbubbles improved the survival of rabbits with VX2 liver tumor
| Autor: | Jianping Gong, Chang-an Liu, Sheng-Wei Li, Zuo-jin Liu, Shiji Zhou, Ji-Jian Wang, Guo-Bing Yin |
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| Rok vydání: | 2012 |
| Předmět: |
Pathology
medicine.medical_specialty Liver tumor business.industry medicine.medical_treatment Suicide gene medicine.disease High-intensity focused ultrasound Terminal deoxynucleotidyl transferase In vivo Apoptosis Thymidine kinase Drug Discovery Immunology Genetics medicine Microbubbles Molecular Medicine business Molecular Biology Genetics (clinical) |
| Zdroj: | The Journal of Gene Medicine. 14:570-579 |
| ISSN: | 1099-498X |
| DOI: | 10.1002/jgm.2668 |
| Popis: | Background To explore the anti-tumor effect of high-intensity focused ultrasound (HIFU) combined with herpes simplex virus thymidine kinase (HSV-TK) gene-loaded ultrasound-targeted microbubbles on VX2 rabbit liver tumors. Methods Seventy-five New Zealand white rabbits were randomly divided into five groups after the models of VX2 rabbit liver tumors were established: (a) HIFU group; (b) HIFU and HSV-TK group (HIFU + HSV-TK); (c) HIFU, HSV-TK and ultrasound group (HIFU + HSV-TK + US); (d) HIFU, HSV-TK gene-loaded microbubbles and ultrasound group (HIFU + HSV-TK-MBs + US); and (e) HSV-TK gene-loaded microbubbles and ultrasound group (HSV-TK-MBs + US). After 2 weeks of VX2 liver tumor implantation, rabbits in groups (a), (b), (c) and (d) received HIFU to establish rabbit models of residual tumor by ablating 80% of the tumor volume. After HIFU ablation, rabbits in different groups received MBs wrapped around HSV-TK or HSV-TK solution via marginal ear veins and/or local ultrasonic irradiation to the tumor. Six rabbits in each group were sacrificed 48 h after the corresponding treatment, and tumors were extracted for in vitro experiments. Thymidine kinase mRNA was detected by the real-time polymerase chain reaction. The green fluorescent protein expression in liver tumor was detected by western blotting and immunohistochemistry. Tumor cell apoptosis was detected by terminal deoxynucleotidyl transferase dUTP nick end labeling. The growth curves of VX2 liver tumors and survival curves of rabbits were compared. Results Forty-eight hours after treatment, TK mRNA and protein were the highest in the HIFU + HSV-TK + US + MBs group and the HSV-TK + US + MBs group (p |
| Databáze: | OpenAIRE |
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