The Antiviral Agent Hypericin hasin vitroActivity against HSV-1 Through Non-Specific Association with Viral and Cellular Membranes
| Autor: | James A. North, Byron K. Murray, Norbert D. Weber, S. G. Wood |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
0301 basic medicine
biology 030106 microbiology General Medicine biology.organism_classification medicine.disease_cause 01 natural sciences Virology In vitro Herpesviridae Virus 0104 chemical sciences Hypericin 010404 medicinal & biomolecular chemistry 03 medical and health sciences chemistry.chemical_compound Herpes simplex virus Mechanism of action chemistry Cell culture Alphaherpesvirinae medicine medicine.symptom |
| Zdroj: | Antiviral Chemistry and Chemotherapy. 5:83-90 |
| ISSN: | 2040-2066 |
| DOI: | 10.1177/095632029400500204 |
| Popis: | The antiviral and virucidal compound, hypericin, was studied regarding its activity and possible mechanism against herpes simplex virus (HSV-1). It was determined that hypericin caused slight inhibition of viral adsorption to and penetration of Vero cells. Additionally, yield reduction assays suggested that hypericin was most effective against HSV-1 as a virucidal agent rather than as an intracellular antiviral agent. Fluorescence microscopy revealed that hypericin initially associated with cytoplasmic membranes and that over the course of time it became concentrated in intracellular membranous regions, probably the Golgi apparatus or endoplasmic reticulum (ER). These concentration events failed to inhibit glycosylation of either viral or cellular proteins and were effectively blocked by compounds which inhibit endocytosis or membrane cycling between the ER and Golgi. Based on fluorescence studies, it was determined that hypericin had non-specific affinity for protein and higher affinity for detergent and lipid. The evidence suggested that strong, non-specific association with membranes, both viral and cellular, are probably the basis of hypericin's virucidal and antiviral activity. |
| Databáze: | OpenAIRE |
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