9α-Hydroxylation of 4-androstene-3,17-dione by resting Rhodococcus sp. cells

Autor:	B. Angelova, I. Dimova, L. Boyadjieva, S. Mutafov, Tatyana Avramova
Rok vydání:	1996
Předmět:	Chromatography Chemistry medicine.medical_treatment Biomass chemistry.chemical_element Substrate (chemistry) Bioengineering Applied Microbiology and Biotechnology Biochemistry Nitrogen Steroid Hydroxylation chemistry.chemical_compound Transformation (genetics) Nutrient medicine Carbon
Zdroj:	Process Biochemistry. 31:179-184
ISSN:	1359-5113
Popis:	The effect of nitrogen and carbon nutrition, growth and biomass storage on the effectiveness of microbial 9α-hydroxylation of 4-androstene-3,17-dione was studied. The transformation was carried out by resting Rhodococcus sp. cells in nutrient deficient transformation medium at different pHs as well as in the presence of several solvents of the steroid compounds. A conversion ratio of ca 0·75 was observed with stationary phase cells grown on medium containing glucose and non-dehydrated casein hydrolysate. A strong influence of the nitrogen source on the effectiveness of the process was noticed. The presence of different alcohols in the reaction medium was found to increase the substrate conversion ratio to ca 0·85. A decrease in the transformation ability of stored cells was a logarithmic function of the storage time.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::642628bfad0ea27fc2774182ae947f52 https://doi.org/10.1016/0032-9592(95)00046-1 Zobrazit plný text záznamu Full Text from ScienceDirect