A three phase hollow fiber liquid-phase microextraction for quantification of lamotrigine in plasma of epileptic patients by capillary electrophoresis
| Autor: | Cristiane Masetto de Gaitani, Regina Helena Costa Queiroz, Luiza Saldanha Ribeiro Barros, Anderson Rodrigo Moraes de Oliveira, Daniel Blascke Carrão |
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| Rok vydání: | 2016 |
| Předmět: |
Detection limit
Analyte Chromatography 010405 organic chemistry Sodium 010401 analytical chemistry Clinical Biochemistry chemistry.chemical_element Hydrochloric acid Phosphate 01 natural sciences Biochemistry Buffer (optical fiber) 0104 chemical sciences Analytical Chemistry chemistry.chemical_compound Capillary electrophoresis Membrane chemistry |
| Zdroj: | ELECTROPHORESIS. 37:2678-2684 |
| ISSN: | 0173-0835 |
| Popis: | A three phase hollow fiber liquid-phase microextraction technique combined with capillary electrophoresis was developed to quantify lamotrigine (LTG) in plasma samples. The analyte was extracted from 4.0 mL of a basic donor phase (composed of 0.5 mL of plasma and 3.5 mL of sodium phosphate solution pH 9.0) through a supported liquid membrane composed of 1-octanol immobilized in the pores of the hollow fiber, and to an acidic acceptor phase (hydrochloric acid solution pH 4.0) placed in the lumen of the fiber. The extraction was carried out for 30 min at 500 rpm. The eletrophoretic analysis was carried out in 130 mmol/L MES buffer, pH 5.0 with a constant voltage of +15 kV and 20°C. Sample injections were performed for 10 s, at a pressure of 0.5 psi. The detection was performed at 214 nm for both LTG and the internal standard lidocaine. Under the optimized conditions, the method showed a limit of quantification of 1.0 μg/mL and was linear over the plasmatic concentration range of 1.0-20.0 μg/mL. Finally, the validated method was applied for the quantification of LTG in plasma samples of epileptic patients. |
| Databáze: | OpenAIRE |
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